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hide">Flow Cytometry & Cell Culture </h2> <div class=""> <div class="content block-content"> <div class="breadcrumb-links"> <div class="content-inner"> <nav class="breadcrumb " role="navigation" aria-labelledby="system-breadcrumb"> <h2 id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/">Home</a> <span class=""> / </span> </li> <li> <a href="/research">Research</a> <span class=""> / </span> </li> <li> <a href="/research/translational-institute-medicine">Translational Institute of Medicine (TIME)</a> <span class=""> / </span> </li> <li> <a href="/research/translational-institute-medicine/research-services">Research Services</a> <span class=""> / </span> </li> <li> <a href="/research/translational-institute-medicine/research-services/core-facility">Core Facility</a> <span class=""> / </span> </li> <li> </li> <li> Flow Cytometry & Cell Culture </li> <li></li> </ol> </nav> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div role="main" class="main main-page"> <div class="clearfix"></div> <div class="clearfix"></div> <div class="clearfix"></div> <div id="content" class="content content-full"> <div class="container"> <div class="content-main-inner"> <div class="row"> <div id="page-main-content" class="main-content col-xs-12 col-md-8 sb-r "> <div class="main-content-inner"> <div class="content-main"> <div class="region region-content"> <div id="block-gavias-financial-content" class="block block-system block-system-main-block no-title"> <div class="content block-content"> <!-- Start Display article for detail page --> <article data-history-node-id="1687" typeof="schema:WebPage" class="node node--type-page node--view-mode-full"> <header> <div class="container"> <div class="widget gsc-heading align-left style-1 small text-dark "> <h1 class="title"><span><span property="schema:name" class="field field--name-title field--type-string field--label-hidden">Flow Cytometry & Cell Culture</span> </span></h1> <div class="line"><span class="one"></span><span class="second"></span><span class="three"></span></div> </div> </div> </header> <div class="node__content clearfix"> <div property="schema:text" class="clearfix text-formatted field field--name-body field--type-text-with-summary field--label-hidden field__item"><p>The TIME Core Flow Cytometry and Cell Culture Laboratory provides an integrated platform for high-resolution cellular analysis and advanced in vitro experimentation.</p> <p>Led by Curtis Noordhof and Dr. Patricia Lima, the facility supports research across immunology, cardiovascular biology, oncology and translational medicine through advanced flow cytometry, cell sorting and cell culture services.</p> <h2>Flow Cytometry</h2> <p>The laboratory is equipped with advanced instrumentation for high-dimensional cellular analysis, phenotyping and complex cell-sorting workflows.</p> <p><strong>Equipment includes:</strong></p> <ul> <li>Sony ID7000 Spectral Cell Analyzer</li> <li>Sony SH800 Cell Sorter</li> <li>Sony MA900 Cell Sorter</li> <li>Beckman Coulter CytoFLEX nano for nanoparticle quantification and cytometry</li> </ul> <h3>Featured instrument: Sony ID7000 Spectral Cell Analyzer</h3> <p>The Sony ID7000 is a high-parameter spectral flow cytometry system capable of detecting more than 40 colours. Its diffraction-grating optical system captures continuous spectral data, helping improve signal separation, reduce spectral overlap and support complex multiparameter analysis.</p> <p><strong>Key features:</strong></p> <ul> <li>Six spatially separated lasers: 320, 355, 405, 488, 561 and 637 nm</li> <li>184 PMT detectors</li> <li>Approximately 10 nm spectral resolution</li> <li>Sensitive side-scatter detection for particles down to approximately 80 nm</li> <li>Standardization mode to support reproducible results across instruments and days</li> <li>Spectral Reference Library to reduce the need for repeated single-colour controls</li> <li>Integrated Autofluorescence Finder</li> <li>Live spectral unmixing</li> <li>Spectral Reference Adjuster</li> <li>Automated clog detection and self-cleaning probe</li> <li>Autosampler supporting tubes, 96-well plates and 384-well plates</li> </ul> <p><a href="https://resources.sonybiotechnology.com/Brochures/Sony_ID7000_Spectral_Cell_Analyzer_Brochure.pdf">View the Sony ID7000 brochure</a></p> <h2>Cell Culture</h2> <p>The laboratory also provides a fully equipped cell culture environment supporting routine and advanced experimental workflows.</p> <p><strong>Services include:</strong></p> <ul> <li>Custom cell culture services</li> <li>Real-time proliferation and viability assays using the Agilent xCELLigence RTCA system</li> <li>Transfection using the Lonza Nucleofector</li> </ul> <h2>Planning your experiment</h2> <h3>Need help designing your flow cytometry experiment?</h3> <p>Contact the TIME Core team before beginning your study. Understanding your research goals allows the team to recommend the most appropriate instrumentation and workflow and provide support with panel design.</p> <p>Patricia Lima: <a href="mailto:pdal@queensu.ca">pdal@queensu.ca</a><br><br> Curtis Noordhof: <a href="mailto:noordhof@queensu.ca">noordhof@queensu.ca</a></p> </div> </div> </article> </div> </div> </div> </div> </div> </div> <!-- Sidebar Left --> <!-- End Sidebar Left --> <!-- Sidebar Right --> <div class="col-lg-4 col-md-4 col-sm-12 col-xs-12 sidebar sidebar-right theiaStickySidebar"> <div class="sidebar-inner"> <div class="region region-sidebar-right"> <nav role="navigation" aria-labelledby="block-mainnavigation-28-menu" id="block-mainnavigation-28" class="block block-menu navigation menu--main"> <h2 id="block-mainnavigation-28-menu"><span><a href="/research/translational-institute-medicine/research-services/core-facility" class="menu-item--active-trail" data-drupal-link-system-path="node/1684">Core Facility</a></span></h2> 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