Flow Cytometry and Cell Culture

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QCPU Room 1610A

Laboratory Scientists: Dr. Patricia Lima (maternity leave); Curtis Noordhof

QCPU's Sony SH800 is a micro fluidic chip-based designed cell sorter which is equipped with 4 lasers (405 nm, 488nm, 561nm and 638nm) that are combined into a single collinear beam. This Sony sorter can detect up to 6 colors plus two scatter parameters. Additionally, the machine is equipped with a Class-II Biosafety cabinet that maintains a sterile environment for the possibility to run BSL-2 sorts

What probes can we run?
What cells can we sort?

Laser	Probe	Peak emission wavelength (nm)	Channel (Filter)
405 nm	BV421	421	FL1 (450/50)
	AF405	421	FL1 (450/50)
	DAPI	455	FL1 (450/50)
	Pacific Blue	455	FL1 (450/50)
	mCFP	475	FL1 (450/50)
	Hoechst 33342	483	FL1 (450/50)
	AmCyan1	491	FL2 (525/50)
	T-Sapphire	511	FL2 (525/50)
	Qdot 525	525	FL2 (525/50)
	Qdot 545	545	FL2 (525/50)
	Pacific Orange	551	FL2 (525/50)
	BV570	570	FL3 (600/60)
	Qdot 585	588	FL3 (600/60)
	Qdot 605	603	FL3 (600/60)
	Qdot 655	654	FL4 (665/30)
	Qdot 705	705	FL5 (720/60)
	Qdot 800	800	FL6 (785/60)
488 nm	EGFP	508	FL2 (525/50)
	CFSE	517	FL2 (525/50)
	FITCI	518	FL2 (525/50)
	AF488	519	FL2 (525/50)
	EYFP	527	FL2 (525/50)
	mCitrine	529	FL2 (525/50)
	PE	576	FL3 (600/60)
	PE-Texas Red	615	FL3 (600/60)
	PI	617	FL3 (600/60)
	7-AAD	647	FL4 (665/30)
	PE-Cy5	670	FL4 (665/30)
	PerCP	675	FL4 (665/30)
	PE-Cy5.5	695	FL5 (720/60)
	PerCP-Cy5.5	695	FL5 (720/60)
	PerCP-eFlour 710	710	FL5 (720/60)
	PE-Cy7	779	FL6 (785/60)
561 nm	mOrange	562	FL3 (600/60)
	DsRed-Monomer	586	FL3 (600/60)
	tdTomato	581	FL3 (600/60)
	mCherry	610	FL3 (600/60)
	mPlum	649	FL4 (665/30)
638 nm	APC	660	FL4 (665/30)
	Cy5	666	FL4 (665/30)
	AF647	668	FL4 (665/30)
	APC-Cy5.5	695	FL5 (720/60)
	AF700	719	FL5 (720/60)
	APC-AF750	775	FL6 (785/60)
	APC-Cy7	779	FL6 (785/60)

Dont see your probe name? Email us

Available Sorting Chip sizes 70µm, 100µm, 130µm.

70µm

Can sort 4 × 10^7 total cells per hour, collection volume: ~10^6 cells in 1mL
Requires higher pressure (60 psi), not ideal for larger or fragile cells

100µm-130µm

Can sort 2 × 10^7 cells/hour, collection volume is ~2.5 × 10^5 cells in 1 mL
Best for large or delicate cells because it uses lower pressure (20psi)
Examples include stem cells, fibroblasts, neurons, and larger cancer cells

*Average cell diameter for different cell types and recommended nozzle size

Cell Type	Average Volume	Average Diameter	Recommended Nozzle
	(um3)	(um)	size (um)
Sperm Cells	30	3.86	70
RBC's	100	5.76	70
Lymphocytes	130	6.29	70
Neutrophils	300	8.31	70
Beta Cells	1000	12.41	70
Fibroblasts	2000	15.63	85
Hela Cells	3000	17.9	100
Osteoblasts	4000	19.7	130
Alveolar Macrophage	5000	21.22	130

Possibility to sort up to 2 populations at the same time:
• Sample area can be cooled and heated.
• The collection area can be cooled.
• Several sort collection options:
15mL, 5mL, 2mL and 1.5mL tubes (2-way sort)
384-well and 96-well (1-way sort)
PCR tubes/strips/plates (1-way sort)
slides
• Sort setup modes: Purity, Single Cell (cloning), Enrichment mode.

This webpage is currently undergoing maintenance! If you want more information please contact us

QCPU Room 1610A

Technical