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QCPU Room 1610A
Laboratory Scientists: Dr. Patricia Lima (maternity leave); Curtis Noordhof
QCPU's Sony SH800 is a micro fluidic chip-based designed cell sorter which is equipped with 4 lasers (405 nm, 488nm, 561nm and 638nm) that are combined into a single collinear beam. This Sony sorter can detect up to 6 colors plus two scatter parameters. Additionally, the machine is equipped with a Class-II Biosafety cabinet that maintains a sterile environment for the possibility to run BSL-2 sorts
| Laser | Probe | Peak emission wavelength (nm) | Channel (Filter) |
| 405 nm | BV421 | 421 | FL1 (450/50) |
| AF405 | 421 | FL1 (450/50) | |
| DAPI | 455 | FL1 (450/50) | |
| Pacific Blue | 455 | FL1 (450/50) | |
| mCFP | 475 | FL1 (450/50) | |
| Hoechst 33342 | 483 | FL1 (450/50) | |
| AmCyan1 | 491 | FL2 (525/50) | |
| T-Sapphire | 511 | FL2 (525/50) | |
| Qdot 525 | 525 | FL2 (525/50) | |
| Qdot 545 | 545 | FL2 (525/50) | |
| Pacific Orange | 551 | FL2 (525/50) | |
| BV570 | 570 | FL3 (600/60) | |
| Qdot 585 | 588 | FL3 (600/60) | |
| Qdot 605 | 603 | FL3 (600/60) | |
| Qdot 655 | 654 | FL4 (665/30) | |
| Qdot 705 | 705 | FL5 (720/60) | |
| Qdot 800 | 800 | FL6 (785/60) | |
| 488 nm | EGFP | 508 | FL2 (525/50) |
| CFSE | 517 | FL2 (525/50) | |
| FITCI | 518 | FL2 (525/50) | |
| AF488 | 519 | FL2 (525/50) | |
| EYFP | 527 | FL2 (525/50) | |
| mCitrine | 529 | FL2 (525/50) | |
| PE | 576 | FL3 (600/60) | |
| PE-Texas Red | 615 | FL3 (600/60) | |
| PI | 617 | FL3 (600/60) | |
| 7-AAD | 647 | FL4 (665/30) | |
| PE-Cy5 | 670 | FL4 (665/30) | |
| PerCP | 675 | FL4 (665/30) | |
| PE-Cy5.5 | 695 | FL5 (720/60) | |
| PerCP-Cy5.5 | 695 | FL5 (720/60) | |
| PerCP-eFlour 710 | 710 | FL5 (720/60) | |
| PE-Cy7 | 779 | FL6 (785/60) | |
| 561 nm | mOrange | 562 | FL3 (600/60) |
| DsRed-Monomer | 586 | FL3 (600/60) | |
| tdTomato | 581 | FL3 (600/60) | |
| mCherry | 610 | FL3 (600/60) | |
| mPlum | 649 | FL4 (665/30) | |
| 638 nm | APC | 660 | FL4 (665/30) |
| Cy5 | 666 | FL4 (665/30) | |
| AF647 | 668 | FL4 (665/30) | |
| APC-Cy5.5 | 695 | FL5 (720/60) | |
| AF700 | 719 | FL5 (720/60) | |
| APC-AF750 | 775 | FL6 (785/60) | |
| APC-Cy7 | 779 | FL6 (785/60) |
Dont see your probe name? Email us
Available Sorting Chip sizes 70µm, 100µm, 130µm.
70µm
- Can sort 4 × 10^7 total cells per hour, collection volume: ~10^6 cells in 1mL
- Requires higher pressure (60 psi), not ideal for larger or fragile cells
100µm-130µm
- Can sort 2 × 10^7 cells/hour, collection volume is ~2.5 × 10^5 cells in 1 mL
- Best for large or delicate cells because it uses lower pressure (20psi)
- Examples include stem cells, fibroblasts, neurons, and larger cancer cells
*Average cell diameter for different cell types and recommended nozzle size
|
Cell Type |
Average Volume |
Average Diameter |
Recommended Nozzle |
|
(um3) |
(um) |
size (um) |
|
|
Sperm Cells |
30 |
3.86 |
70 |
|
RBC's |
100 |
5.76 |
70 |
|
Lymphocytes |
130 |
6.29 |
70 |
|
Neutrophils |
300 |
8.31 |
70 |
|
Beta Cells |
1000 |
12.41 |
70 |
|
Fibroblasts |
2000 |
15.63 |
85 |
|
Hela Cells |
3000 |
17.9 |
100 |
|
Osteoblasts |
4000 |
19.7 |
130 |
|
Alveolar Macrophage |
5000 |
21.22 |
130 |
Possibility to sort up to 2 populations at the same time:
• Sample area can be cooled and heated.
• The collection area can be cooled.
• Several sort collection options:
15mL, 5mL, 2mL and 1.5mL tubes (2-way sort)
384-well and 96-well (1-way sort)
PCR tubes/strips/plates (1-way sort)
slides
• Sort setup modes: Purity, Single Cell (cloning), Enrichment mode.