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Flow Cytometry and Cell Culture

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QCPU Room 1610A

Laboratory Scientists: Dr. Patricia Lima (maternity leave); Curtis Noordhof 

QCPU's Sony SH800 is a micro fluidic chip-​based designed cell sorter which is equipped with 4 lasers (405 nm, 488nm, 561nm and 638nm) that are combined into a single collinear beam. This Sony sorter can detect up to 6 colors plus two scatter parameters. Additionally, the machine is equipped with a Class-​II Biosafety cabinet that maintains a sterile environment for the possibility to run BSL-​2 sorts

Technical

Laser Probe Peak emission wavelength (nm) Channel (Filter)
405 nm  BV421 421 FL1 (450/50)
  AF405 421 FL1 (450/50)
  DAPI 455 FL1 (450/50)
  Pacific Blue 455 FL1 (450/50)
  mCFP 475 FL1 (450/50)
  Hoechst 33342 483 FL1 (450/50)
  AmCyan1 491 FL2 (525/50)
  T-Sapphire 511 FL2 (525/50)
  Qdot 525 525 FL2 (525/50)
  Qdot 545 545 FL2 (525/50)
  Pacific Orange 551 FL2 (525/50)
  BV570 570 FL3 (600/60)
  Qdot 585 588 FL3 (600/60)
  Qdot 605 603 FL3 (600/60)
  Qdot 655 654 FL4 (665/30)
  Qdot 705 705 FL5 (720/60)
  Qdot 800 800 FL6 (785/60)
488 nm EGFP 508 FL2 (525/50)
  CFSE 517 FL2 (525/50)
  FITCI 518 FL2 (525/50)
  AF488 519 FL2 (525/50)
  EYFP 527 FL2 (525/50)
  mCitrine 529 FL2 (525/50)
  PE 576 FL3 (600/60)
  PE-Texas Red 615 FL3 (600/60)
  PI 617 FL3 (600/60)
  7-AAD 647 FL4 (665/30)
  PE-Cy5 670 FL4 (665/30)
  PerCP 675 FL4 (665/30)
  PE-Cy5.5 695 FL5 (720/60)
  PerCP-Cy5.5 695 FL5 (720/60)
  PerCP-eFlour 710 710 FL5 (720/60)
  PE-Cy7 779 FL6 (785/60)
561 nm mOrange 562 FL3 (600/60)
  DsRed-Monomer 586 FL3 (600/60)
  tdTomato 581 FL3 (600/60)
  mCherry 610 FL3 (600/60)
  mPlum 649 FL4 (665/30)
638 nm APC 660 FL4 (665/30)
  Cy5 666 FL4 (665/30)
  AF647 668 FL4 (665/30)
  APC-Cy5.5 695 FL5 (720/60)
  AF700 719 FL5 (720/60)
  APC-AF750 775 FL6 (785/60)
  APC-Cy7 779 FL6 (785/60)

Dont see your probe name?  Email us

Available Sorting Chip sizes 70µm, 100µm, 130µm.

70µm

  • Can sort 4 × 10^7 total cells per hour, collection volume: ~10^6 cells in 1mL
  • Requires higher pressure (60 psi), not ideal for larger or fragile cells

100µm-130µm

  • Can sort 2 × 10^7 cells/hour, collection volume is ~2.5 × 10^5 cells in 1 mL
  • Best for large or delicate cells because it uses lower pressure (20psi)
  • Examples include stem cells, fibroblasts, neurons, and larger cancer cells

 

*Average cell diameter for different cell types and recommended nozzle size

Cell Type

Average Volume

Average Diameter

Recommended Nozzle

 

(um3)

(um)

size (um)

Sperm Cells

30

3.86

70

RBC's

100

5.76

70

Lymphocytes

130

6.29

70

Neutrophils

300

8.31

70

Beta Cells

1000

12.41

70

Fibroblasts

2000

15.63

85

Hela Cells

3000

17.9

100

Osteoblasts

4000

19.7

130

Alveolar Macrophage

5000

21.22

130

 Possibility to sort up to 2 populations at the same time:
• Sample area can be cooled and heated.
• The collection area can be cooled.
• Several sort collection options:
15mL, 5mL, 2mL and 1.5mL tubes (2-​way sort)
384-​well and 96-​well (1-​way sort)
    PCR tubes/strips/plates (1-​way sort)
    slides
• Sort setup modes: Purity, Single Cell (cloning), Enrichment mode.